2024년 3월 25일
Improved Neural Inductivity of Size-Controlled 3D Human Embryonic Stem Cells Using Magnetic Nanoparticles
Abstract
- Background: To improve the efficiency of neural development from human embryonic stem cells, human embryoid body (hEB) generation is vital through 3-dimensional formation. However, conventional approaches still have limitations: long-term cultivation and laborious steps for lineage determination.
- Methods: In this study, we controlled the size of hEBs for ectodermal lineage specification using cell-penetrating magnetic nanoparticles (MNPs), which resulted in reduced time required for initial neural induction. The magnetized cells were applied to concentrated magnetic force for magnet-derived multicellular organization. The uniformly sized hEBs were differentiated in neural induction medium (NIM) and suspended condition. This neurally induced MNP-hEBs were compared with other groups.
- Results: As a result, the uniformly sized MNP-hEBs in NIM showed significantly improved neural inductivity through morphological analysis and expression of neural markers. Signaling pathways of the accelerated neural induction were detected via expression of representative proteins; Wnt signaling, dopaminergic neuronal pathway, intercellular communications, and mechanotransduction. Consequently, we could shorten the time necessary for early neurogenesis, thereby enhancing the neural induction efficiency.
- Conclusion: Overall, this study suggests not only the importance of size regulation of hEBs at initial differentiation stage but also the efficacy of MNP-based neural induction method and stimulations for enhanced neural tissue regeneration.
논문정보
- 형식: Research article
- 게재일: 2024년 03월 (BRIC 등록일 2024-03-20)
- 연구진: 국내 연구진
- 분야:바이오·의료융합 > 바이오센싱 및 나노바이오물질
Authors and Affiliations
Boram Son1,2†, Sora Park1†, Sungwoo Cho1, Jeong Ah Kim3, Seung-Ho Baek4, Ki Hyun Yoo5, Dongoh Han5, Jinmyoung Joo6, Hee Ho Park2,7*, and Tai Hyun Park1,8*
1School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
2Department of Bioengineering, Hanyang University, 222 Wangsimri-ro, Seongdong-gu, Seoul 04763, Republic of Korea.
3Center for Scientific Instrumentation, Korea Basic Science Institute, Cheongju, Chungbuk 28119, Republic of Korea.
4Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44429, Korea.
5SIMPLE Planet Inc., 48 Achasan-ro 17-gil, Seongdong-gu, Seoul 04799, Korea.
6Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Republic of Korea.
7Research Institute for Convergence of Basic Science, Hanyang University, Seoul 04763, Republic of Korea.
8Department of Nutritional Science and Food Management, Ewha Womans University, Seodaemun-gu, Seoul 03760, Republic of Korea.
*Address correspondence to: Hee Ho Park, Tai Hyun Park
†These authors contributed equally to this work.
관련 인터뷰
재생의학적 관점에서 한번 손상되면 회복이 어려운 뇌, 심장과 같은 기관의 세포들은 오랜 기간 줄기세포 연구의 주요한 타겟이 되어 왔습니다. 그러나 배아줄기세포(embryonic stem cell, ESC)와 같은 만능성 줄기세포(pluripotent stem cell, PSC)를 의도하는 방향으로 분화 유도하고 나아가 신경세포와 같은 특정 세포 종류로 성숙시키는 데에는 여러가지 어려움이 따릅니다. 번거로운 단계 및 과정에도 불구하고 낮은 분화 효율은 극복해야 하는 요인 중 하나입니다.